12284 Mtus2 Status: ES cell colonies screened / QC no positives
| Gene | Mtus2 |
| Chromosome | 5 |
| Deletion Start | 148,076,402 |
| Deletion End | 148,107,193 |
| Deletion Size | 30,792 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 148,077,166 | 148,077,187 | GCCACATGGCCTGGATGTTTAC |
| TUP | 148,077,190 | 148,077,216 | CGAGCGCATCACTCATGCAGAATTGAC |
| TUR | 148,077,220 | 148,077,239 | TCGACGCTGAAGCTGAACTG |
| TDF | 148,106,344 | 148,106,362 | AGCTTGTCCCAGGCTGTTG |
| TDP | 148,106,363 | 148,106,385 | TGACTCTCCCTCCTGGGTCCTCT |
| TDR | 148,106,386 | 148,106,409 | CCTAACTTACAAGGGCAGGACTTC |
PCR Primers
| Primer | Sequence |
| SU | GCATCCCTTTGATGCTTCC |
| SD | AAGGAGGGTCAAATAGGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
