| Gene | Vsig10 |
| Chromosome | 5 |
| Deletion Start | 117,319,441 |
| Deletion End | 117,352,803 |
| Deletion Size | 33,363 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 117,319,682 | 117,319,702 | TGTGTCCATCTCACTGTCTCC |
| TUP | 117,319,704 | 117,319,727 | CGCTTCCCAGATTGACCCGGCCTT |
| TUR | 117,319,772 | 117,319,791 | GGGTCGGCTCAGGTAAACTC |
| TDF | 117,352,709 | 117,352,727 | CCTGCCGGTGAGATCACTC |
| TDP | 117,352,731 | 117,352,756 | TCCATCTCTTGCACCGACAGATGACA |
| TDR | 117,352,780 | 117,352,799 | GGCTTGCCATCCTCTTGAAC |
PCR Primers
| Primer | Sequence |
| SU | GGAGAACCCAGATGACAAGC |
| SD | GTAGAGTCAGTATCACAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12286A-C2 | VGB6 | C57BL/6NTac | |
| 12286D-E12 | VGB6 | C57BL/6NTac | |
