12289 Hspb6 Status: Cancelled
| Gene | Hspb6 |
| Chromosome | 7 |
| Deletion Start | 30,553,338 |
| Deletion End | 30,554,659 |
| Deletion Size | 1,322 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,553,349 | 30,553,366 | GCCTGTGCAGCCTTCTTG |
| TUP | 30,553,369 | 30,553,390 | TGCGCCGTGCTTCAGCTCCTTT |
| TUR | 30,553,418 | 30,553,436 | GCCGAAACGCTGGTCAAAG |
| TDF | 30,554,556 | 30,554,575 | TCTCCTGAGGGTGTCCTGTC |
| TDP | 30,554,578 | 30,554,597 | TCCAGGCCACACCAGCGTCG |
| TDR | 30,554,607 | 30,554,627 | AGCAGGTGGTGACGGAAGTTG |
PCR Primers
| Primer | Sequence |
| SU | TGGCGGAGATGGATTAAGAG |
| SD | GACTGGTGGACTAGGCTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
