12304 Tmod4 Status: Cancelled
| Gene | Tmod4 |
| Chromosome | 3 |
| Deletion Start | 95,125,558 |
| Deletion End | 95,129,211 |
| Deletion Size | 3,654 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,125,852 | 95,125,875 | TGACCAGACAAAGAAGAGTCCAAC |
| TUP | 95,125,876 | 95,125,898 | AGGGCCACTGGACAGAGATGCCC |
| TUR | 95,125,941 | 95,125,961 | TGTAGGGCACCAAGTCATCAC |
| TDF | 95,128,608 | 95,128,626 | GCCTGGTGATGCGGTAGAG |
| TDP | 95,128,627 | 95,128,652 | ATGGAGATGGCCACTGTGCTAGAGCA |
| TDR | 95,128,659 | 95,128,678 | ACCCGAAGCGGACAATAGAC |
PCR Primers
| Primer | Sequence |
| SU | CTTTCAATAGGAACTGGGCG |
| SD | CTGTCAGAAAGAGGTCGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
