12306 Anapc11 Status: Cancelled
| Gene | Anapc11 |
| Chromosome | 11 |
| Deletion Start | 120,599,263 |
| Deletion End | 120,605,442 |
| Deletion Size | 6,180 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,599,304 | 120,599,325 | CTGGGTAGCCAATGATGAGAAC |
| TUP | 120,599,328 | 120,599,350 | CGGCATCTGCAGGATGGCGTTTA |
| TUR | 120,599,373 | 120,599,392 | GAGAGCGCATAGGAAGACAC |
| TDF | 120,605,003 | 120,605,024 | GGTGTGAGAAAGGGACCAGAAC |
| TDP | 120,605,026 | 120,605,045 | TAGTGGGCCAGGCCTCTCCC |
| TDR | 120,605,115 | 120,605,135 | AGGGCTGTGACCCAAATCATC |
PCR Primers
| Primer | Sequence |
| SU | GAAGGCTCGGTCTCCTGAC |
| SD | GCCTGAATCTCTCCTCCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 133 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
