12310 Thoc6 Status: Cancelled
| Gene | Thoc6 |
| Chromosome | 17 |
| Deletion Start | 23,673,549 |
| Deletion End | 23,668,795 |
| Deletion Size | 4,755 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,672,952 | 23,672,973 | CTTCTGTTCTTCCTGCCTTCAC |
| TUP | 23,672,925 | 23,672,949 | CCACTGAGTGCTCCAGGCTATTGGG |
| TUR | 23,672,883 | 23,672,904 | CGTTTGAGCACCAGCATTGTAG |
| TDF | 23,669,465 | 23,669,485 | TCAGATGCCCTCACGTCTTTC |
| TDP | 23,669,441 | 23,669,463 | TCCTTTGCAGGAGTGCTCGAGGC |
| TDR | 23,669,379 | 23,669,397 | TGCCCAGCTTACCATCCAG |
PCR Primers
| Primer | Sequence |
| SU | CAGGGTCCTGCAGATAGAGG |
| SD | AAGAGGTGGACAGAGGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
