12312 Zfp446 Status: Cancelled
| Gene | Zfp446 |
| Chromosome | 7 |
| Deletion Start | 12,977,833 |
| Deletion End | 12,982,672 |
| Deletion Size | 4,840 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 12,978,482 | 12,978,505 | GTAGTAAGCTGAGGCAGTATGAGG |
| TUP | 12,978,509 | 12,978,532 | CCACAATTCAGCTGCGATCAGCGA |
| TUR | 12,978,534 | 12,978,552 | TATGGGTTGGGAGGCACAG |
| TDF | 12,982,237 | 12,982,259 | TGCAGATCCAAGGCTAGTTCAAG |
| TDP | 12,982,261 | 12,982,284 | CACTCCAAGTGAAAGGCAGAGCCC |
| TDR | 12,982,299 | 12,982,318 | TGGTGTGGGAGACAAGACTG |
PCR Primers
| Primer | Sequence |
| SU | TTAACGGAGATCCGATGGAG |
| SD | AAGGCAACTGTGGTTTCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
