12318 Tbx20 Status: Cancelled
| Gene | Tbx20 |
| Chromosome | 9 |
| Deletion Start | 24,773,842 |
| Deletion End | 24,750,317 |
| Deletion Size | 23,526 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,773,789 | 24,773,807 | TCGAGCCAATGCCTTCTCC |
| TUP | 24,773,766 | 24,773,788 | ATCGCCGCGCTTATGTCCAGCGG |
| TUR | 24,773,736 | 24,773,755 | TTCTCTGCTGCCTCCTTCTC |
| TDF | 24,750,571 | 24,750,589 | GCCTGAAAGGCCACCAAAC |
| TDP | 24,750,537 | 24,750,564 | TGTCTCAGTCATGGACCAGTATCCCTGT |
| TDR | 24,750,513 | 24,750,536 | GCTATCTGAGTGAGGGACAAGTTC |
PCR Primers
| Primer | Sequence |
| SU | CTGCAGAGAGGAGATCCGAC |
| SD | AAGTGCCAATCAGTAAGACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
