12321 F10 Status: Cancelled
| Gene | F10 |
| Chromosome | 8 |
| Deletion Start | 13,037,841 |
| Deletion End | 13,055,882 |
| Deletion Size | 18,042 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 13,038,606 | 13,038,625 | TCGAGTCAGGACAGTTGGAG |
| TUP | 13,038,627 | 13,038,649 | CCTTGCTGGGTTCCGTGGACAGT |
| TUR | 13,038,668 | 13,038,687 | TTGGCAGCCCAGAACTAAAG |
| TDF | 13,055,311 | 13,055,333 | AGGAAGAAGGCAACGAGATGGTG |
| TDP | 13,055,334 | 13,055,359 | CACGAGGTGGACGTGGTCATTAAGCA |
| TDR | 13,055,366 | 13,055,385 | CGTAGGTGTCCCTCTGAAAC |
PCR Primers
| Primer | Sequence |
| SU | CTACCAGCTGGGTAAGTGCC |
| SD | AGAGACCTTTGCTCACTTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
