12323 Sftpc Status: Cancelled
| Gene | Sftpc |
| Chromosome | 14 |
| Deletion Start | 70,524,040 |
| Deletion End | 70,521,326 |
| Deletion Size | 2,715 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,523,150 | 70,523,169 | TGAAGCTGGCACATGGACTG |
| TUP | 70,523,120 | 70,523,146 | TGAGAAGGAACAAAGGAGACCCGTGTA |
| TUR | 70,523,094 | 70,523,111 | TCCAGGTAGCCCTGTCTC |
| TDF | 70,521,653 | 70,521,671 | TGTGTCCTGCCATCCCTAC |
| TDP | 70,521,631 | 70,521,652 | CAGCTCTCAGGTGGCCCTGCTA |
| TDR | 70,521,611 | 70,521,629 | CTCAGCCAAGGCCAACAAC |
PCR Primers
| Primer | Sequence |
| SU | AGAGGATTCATGTGCCTAGG |
| SD | CTGCTAGAAGCCGAGCTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 61 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
