12325 Mefv Status: Cancelled
| Gene | Mefv |
| Chromosome | 16 |
| Deletion Start | 3,718,059 |
| Deletion End | 3,707,958 |
| Deletion Size | 10,102 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,717,808 | 3,717,826 | GCGCCAACTAGCAGAGGAG |
| TUP | 3,717,784 | 3,717,806 | TTCGCAAAGCCACAGGCACAGGT |
| TUR | 3,717,758 | 3,717,776 | CCCACTGGGAAGAGGCTTG |
| TDF | 3,708,134 | 3,708,155 | TGGGTCCCTGTATGTTCCTATC |
| TDP | 3,708,086 | 3,708,115 | AAATTCCCTCTTCCTTAGATTGGGAGCACC |
| TDR | 3,708,063 | 3,708,081 | GCGGATCTGTGGGCTTCTC |
PCR Primers
| Primer | Sequence |
| SU | AAGCAAGAGCTCAGGAGCAG |
| SD | ATATGGCCCAGGGTTAAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
