12327 Nkx2-5 Status: Cancelled
| Gene | Nkx2-5 |
| Chromosome | 17 |
| Deletion Start | 26,841,352 |
| Deletion End | 26,838,989 |
| Deletion Size | 2,364 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 26,840,584 | 26,840,608 | ACACTGAGAACTGCTAAACCGAAAG |
| TUP | 26,840,554 | 26,840,577 | CCCGTGTATCCTGGGCCTTGTGTA |
| TUR | 26,840,515 | 26,840,533 | TGGCACAAAGGAGGCAAGG |
| TDF | 26,839,859 | 26,839,878 | GGCGCCCGAGATTTCTCTTG |
| TDP | 26,839,827 | 26,839,855 | TGTTTTGTCCAAGCCACTTAGGCATTTCC |
| TDR | 26,839,790 | 26,839,809 | GCTCGCTCGGTTCGATGTTG |
PCR Primers
| Primer | Sequence |
| SU | CAGGCTAACGAGGAGCAATC |
| SD | TGGGAAGAGGGTTCTCATTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
