12337 Bmp15 Status: Cancelled
| Gene | Bmp15 |
| Chromosome | X |
| Deletion Start | 6,320,360 |
| Deletion End | 6,315,566 |
| Deletion Size | 4,795 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,320,286 | 6,320,309 | GAACAGAGGGTCCAAATGGCAAAG |
| TUP | 6,320,256 | 6,320,275 | CCTCCACCGCCCTCCTTGCT |
| TUR | 6,320,236 | 6,320,255 | GAGGGCAATGTAGGGTCGTC |
| TDF | 6,316,497 | 6,316,517 | TGGTCTGCCCTGTTCTTTCAC |
| TDP | 6,316,471 | 6,316,494 | TTGTGCAGGTTCCTGGCATGTACA |
| TDR | 6,316,434 | 6,316,456 | TGCTACCTGGTTTGATGCTAGAG |
PCR Primers
| Primer | Sequence |
| SU | GCCTTTAAATCTGTGGCAGC |
| SD | AGAGCCCAACTGGACACATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
