12339 Acan Status: Cancelled
| Gene | Acan |
| Chromosome | 7 |
| Deletion Start | 79,082,198 |
| Deletion End | 79,114,280 |
| Deletion Size | 32,083 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,082,726 | 79,082,745 | GGAACTCAAGGGCTGAACAG |
| TUP | 79,082,748 | 79,082,771 | TGTGAGACCTCCCAAACAGTGGGA |
| TUR | 79,082,785 | 79,082,804 | CACCATGAGCAAGCCTTCTC |
| TDF | 79,114,171 | 79,114,192 | TGATTTCCCACCTCCCTTTCTC |
| TDP | 79,114,195 | 79,114,217 | TGCAGCCAACACCTACAAGCACA |
| TDR | 79,114,220 | 79,114,242 | GGTCTCATGCTCCGCTTCTGTAG |
PCR Primers
| Primer | Sequence |
| SU | TGGCACATATCAGTAGCTATC |
| SD | CTGGAGGCGAAGTAACCAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
