12345 Foxj1 Status: Cancelled
| Gene | Foxj1 |
| Chromosome | 11 |
| Deletion Start | 116,334,536 |
| Deletion End | 116,331,692 |
| Deletion Size | 2,845 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 116,333,832 | 116,333,856 | GGAGGTACAGACGAAGTTGTAGAAG |
| TUP | 116,333,806 | 116,333,827 | ACCAATAGGGCCCGGTGTAGCC |
| TUR | 116,333,761 | 116,333,780 | TCGGACACGGGACAAGAATG |
| TDF | 116,331,859 | 116,331,879 | CTGCTGACAGCCTGGACTTTG |
| TDP | 116,331,832 | 116,331,857 | TGAGACCTTCTTGGCCACATCCTTCC |
| TDR | 116,331,796 | 116,331,814 | GGCAGCCACTACCACTCTC |
PCR Primers
| Primer | Sequence |
| SU | CCCTGGACTATTGGGATGTG |
| SD | TGAGCGTTTGTTGTACCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
