12346 Fgf23 Status: Cancelled
| Gene | Fgf23 |
| Chromosome | 6 |
| Deletion Start | 127,073,033 |
| Deletion End | 127,080,519 |
| Deletion Size | 7,487 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,073,117 | 127,073,136 | CCATTGCTTGGCTCCAACTG |
| TUP | 127,073,140 | 127,073,164 | AAGCCTGACCCACCTGTACACGGCT |
| TUR | 127,073,173 | 127,073,195 | GGATCTGTAGGTGATAGCTGGTC |
| TDF | 127,080,020 | 127,080,043 | GTTTCCTTTCTCTTACAGCTTCAC |
| TDP | 127,080,046 | 127,080,070 | CAGCCCAGAGAATTGCAAGTTCCGC |
| TDR | 127,080,073 | 127,080,090 | CCATTCTCCAGCGTCCAC |
PCR Primers
| Primer | Sequence |
| SU | AGGTCAACAAATGACCCAGG |
| SD | GAGACCCGGCTAATAAAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
