12347 Mpl Status: Cancelled
| Gene | Mpl |
| Chromosome | 4 |
| Deletion Start | 118,457,468 |
| Deletion End | 118,443,368 |
| Deletion Size | 14,101 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 118,457,378 | 118,457,398 | GCCAAGGTGAGGTGGATAGAG |
| TUP | 118,457,343 | 118,457,369 | TTACCTATTCACAGGCAAAGGGAGCCC |
| TUR | 118,457,301 | 118,457,320 | GAAGGGAACCAGTGAGAGTC |
| TDF | 118,444,249 | 118,444,268 | ACTGTCTTGGCAGAGTGGTC |
| TDP | 118,444,224 | 118,444,248 | AGGCCAACCCGCTTTGACTATGCCC |
| TDR | 118,444,194 | 118,444,212 | TGAGGCAGCGGAGTAAGTG |
PCR Primers
| Primer | Sequence |
| SU | GCGTGGTTAGCACTGTTCTG |
| SD | GGAGGAGGATGCCAAGAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
