| Gene | Serpinf2 |
| Chromosome | 11 |
| Deletion Start | 75,438,451 |
| Deletion End | 75,432,367 |
| Deletion Size | 6,085 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,438,416 | 75,438,436 | CTCCTCGTACTCAGCTTGTCC |
| TUP | 75,438,390 | 75,438,413 | CCTGCAAGGTCCCTGTTTCACGGT |
| TUR | 75,438,356 | 75,438,375 | TGACCACTTCCCACTCAGAC |
| TDF | 75,433,238 | 75,433,257 | GCCAACCTGACCTGGGATAC |
| TDP | 75,433,214 | 75,433,236 | CTGTACCATCCCTCGCTGCAGGA |
| TDR | 75,433,179 | 75,433,200 | GATGGAGTTTAGGCAGCCACAC |
PCR Primers
| Primer | Sequence |
| SU | GTCAGGTGGCTTTGCTAGGG |
| SD | CCTCTCCAGCCTCTGCTTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12349A-B3 | VGB6 | C57BL/6NTac | |
| 12349A-D9 | VGB6 | C57BL/6NTac | |
| 12349A-E10 | VGB6 | C57BL/6NTac | |
