12352 Sh2d1a Status: Cancelled
| Gene | Sh2d1a |
| Chromosome | X |
| Deletion Start | 42,502,726 |
| Deletion End | 42,521,827 |
| Deletion Size | 19,102 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 42,502,998 | 42,503,018 | TTCCAGCTCTGACGTATGGTC |
| TUP | 42,503,019 | 42,503,038 | CCAGGGCTCAGCCCAGGGAT |
| TUR | 42,503,084 | 42,503,104 | CGCACTGGTGAAATGCAGTTG |
| TDF | 42,521,040 | 42,521,063 | GCTTATTGTCCCTCTTCCTCTTTG |
| TDP | 42,521,072 | 42,521,101 | AAATACCACTTTGCTTCAGTAACCCACGCA |
| TDR | 42,521,112 | 42,521,132 | AGCCTTCCTGTGCCATCTTTC |
PCR Primers
| Primer | Sequence |
| SU | GCTTCGAAGTTTGCAGGAAC |
| SD | CATGAAGTCAAGCAAGACGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
