Gene | Tnf |
Chromosome | 17 |
Deletion Start | 35,201,837 |
Deletion End | 35,200,130 |
Deletion Size | 1,708 |
Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 35,201,646 | 35,201,666 | CAAAGGGATGAGGTGAGTGTC |
TUP | 35,201,621 | 35,201,645 | TGGGCAACCCTTATTCTCGCTCACA |
TUR | 35,201,589 | 35,201,610 | CTGTCCTTCTTGCCCTCCTAAC |
TDF | 35,200,483 | 35,200,503 | CCAACGGCATGGATCTCAAAG |
TDP | 35,200,457 | 35,200,481 | CAACCAACTAGTGGTGCCAGCCGAT |
TDR | 35,200,432 | 35,200,454 | CCTGGGAGTAGACAAGGTACAAC |
PCR Primers
Primer | Sequence |
SU | CAACTTTCCAAACCCTCTGC |
SD | CTCCCTTTGCAGAACTCAGG |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
TUF | TUR | 78 bp | + | + | - |
TDF | TDR | 72 bp | + | + | - |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
12361A-A7 | VGB6 | C57BL/6NTac | |
12361A-F12 | VGB6 | C57BL/6NTac | |
12361A-C4 | VGB6 | C57BL/6NTac | |
12361A-E10 | VGB6 | C57BL/6NTac | |
12361A-D5 | VGB6 | C57BL/6NTac | |
12361A-B11 | VGB6 | C57BL/6NTac | |