12361   Tnf     Status: ES cell colonies screened / QC positives

GeneTnf
Chromosome17
Deletion Start35,201,837
Deletion End35,200,130
Deletion Size1,708
Genome Build38
MGI IDMGI:104798
NCBI Gene ID21926
BAC ID126O13
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 17

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF35,201,64635,201,666CAAAGGGATGAGGTGAGTGTC
TUP35,201,62135,201,645TGGGCAACCCTTATTCTCGCTCACA
TUR35,201,58935,201,610CTGTCCTTCTTGCCCTCCTAAC
TDF35,200,48335,200,503CCAACGGCATGGATCTCAAAG
TDP35,200,45735,200,481CAACCAACTAGTGGTGCCAGCCGAT
TDR35,200,43235,200,454CCTGGGAGTAGACAAGGTACAAC

PCR Primers

PrimerSequence
SUCAACTTTCCAAACCCTCTGC
SDCTCCCTTTGCAGAACTCAGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR78 bp++-
TDFTDR72 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
12361A-A7VGB6C57BL/6NTac
12361A-F12VGB6C57BL/6NTac
12361A-C4VGB6C57BL/6NTac
12361A-E10VGB6C57BL/6NTac
12361A-D5VGB6C57BL/6NTac
12361A-B11VGB6C57BL/6NTac