12363 Tnfrsf11b Status: Cancelled
| Gene | Tnfrsf11b |
| Chromosome | 15 |
| Deletion Start | 54,278,244 |
| Deletion End | 54,251,901 |
| Deletion Size | 26,344 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 54,278,208 | 54,278,227 | CGCACTCCTGGTGGTAAGTC |
| TUP | 54,278,181 | 54,278,202 | CAGGAGGCTGCTGACCACCTGG |
| TUR | 54,278,098 | 54,278,119 | AGGCTTCGTGTTCCAACCAAAC |
| TDF | 54,252,377 | 54,252,399 | CGCTTTGGTTCTTGCAGACATTG |
| TDP | 54,252,353 | 54,252,376 | ACCTCTGTGAAAGCAGCGTGCAGC |
| TDR | 54,252,316 | 54,252,336 | AGCTGCTCTGTGGTGAGGTTC |
PCR Primers
| Primer | Sequence |
| SU | GCTGCCTTGTTCTTCAGCTC |
| SD | TTGCCTAGCAACCTACAAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 130 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
