12364 Fbln5 Status: Cancelled
| Gene | Fbln5 |
| Chromosome | 12 |
| Deletion Start | 101,818,747 |
| Deletion End | 101,809,721 |
| Deletion Size | 9,027 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,818,275 | 101,818,296 | CAGAGGGAATGGGACCTAGAAG |
| TUP | 101,818,250 | 101,818,272 | TCGAGCCAATGCACAGTGGACTC |
| TUR | 101,818,226 | 101,818,245 | TGCTTACATGCGTCTCTGTC |
| TDF | 101,809,819 | 101,809,839 | ACATCCTACTCAGGCCCATAC |
| TDP | 101,809,782 | 101,809,807 | CCCACCAGTACCAGCTTCCAACTACC |
| TDR | 101,809,745 | 101,809,764 | TACCCAAAGCGGCAGACAAG |
PCR Primers
| Primer | Sequence |
| SU | CCCAAGATTGTTGTGAGGAG |
| SD | GGTTGGTCACCAATGAGATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
