12367 Col4a4 Status: Cancelled
| Gene | Col4a4 |
| Chromosome | 1 |
| Deletion Start | 82,571,412 |
| Deletion End | 82,541,174 |
| Deletion Size | 30,239 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 82,571,343 | 82,571,366 | GAGTATCCTTGGTGCGTTTATGTC |
| TUP | 82,571,312 | 82,571,341 | TTGTGGAAGACATGTATTTATGTGCTGCCA |
| TUR | 82,571,259 | 82,571,283 | TGAATGCGTTATGGGAAAGATGTTG |
| TDF | 82,541,792 | 82,541,816 | GCAGCTACAGGACATAAAGTCATTG |
| TDP | 82,541,758 | 82,541,785 | TCAAGTCTACCAGGCACTTTCTCCGTCA |
| TDR | 82,541,729 | 82,541,751 | ACCATGTGAGGAACAGAGCTATC |
PCR Primers
| Primer | Sequence |
| SU | GCTATGTTAACAGCCATTGGG |
| SD | AGCATGCTTCTAAGACTTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
