12370 Tbx1 Status: Cancelled
| Gene | Tbx1 |
| Chromosome | 16 |
| Deletion Start | 18,586,936 |
| Deletion End | 18,581,915 |
| Deletion Size | 5,022 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 18,586,587 | 18,586,606 | CTGGAGATGAAGGCGCTGTG |
| TUP | 18,586,559 | 18,586,583 | CGAGTTCAATCAGCTGGGCACCGAG |
| TUR | 18,586,534 | 18,586,552 | ACCTGCCTGCCTTGGTGAC |
| TDF | 18,582,778 | 18,582,796 | CCGCTCGTGAGTGCCTTTG |
| TDP | 18,582,754 | 18,582,775 | CGCTCTCGGAATCCCGTGGCTT |
| TDR | 18,582,697 | 18,582,716 | CCGCGATCCCACAACCTTAG |
PCR Primers
| Primer | Sequence |
| SU | GGAAGAAGTTAGACGGAAGGG |
| SD | CTCACTCCACACCCACACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
