12375 Foxe1 Status: Cancelled
| Gene | Foxe1 |
| Chromosome | 4 |
| Deletion Start | 46,344,197 |
| Deletion End | 46,345,337 |
| Deletion Size | 1,141 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,344,429 | 46,344,450 | GCGGCATCTACAAGTTCATCAC |
| TUP | 46,344,453 | 46,344,475 | AGCGCTTCCCGTTCTACCGCGAC |
| TUR | 46,344,491 | 46,344,509 | TGTGGCGGATGCTGTTCTG |
| TDF | 46,345,141 | 46,345,159 | GGCCACGGTGGACTTCTAC |
| TDP | 46,345,165 | 46,345,184 | CACGTCGCCGGGCCAGTTCG |
| TDR | 46,345,204 | 46,345,224 | AGTTGCCCACCGGGATTGTAG |
PCR Primers
| Primer | Sequence |
| SU | TCGACGGTCATCCTCAGAC |
| SD | ACACGGTGAAGCCTTACGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
