12376 Mylk2 Status: Cancelled
| Gene | Mylk2 |
| Chromosome | 2 |
| Deletion Start | 152,911,551 |
| Deletion End | 152,922,195 |
| Deletion Size | 10,645 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 152,911,662 | 152,911,679 | GTTGGGCAGGCTTCTCAC |
| TUP | 152,911,680 | 152,911,702 | TGGGAAGTCTGTGCCCAAGGTTC |
| TUR | 152,911,704 | 152,911,722 | TCCCTCACGCACCAAACAG |
| TDF | 152,921,230 | 152,921,248 | CCCGAGTGGAACAGGAGAC |
| TDP | 152,921,419 | 152,921,442 | CCAACCCACAGGTTGAGTCACTGC |
| TDR | 152,921,494 | 152,921,511 | GTGCCGTGGCACTGTTTG |
PCR Primers
| Primer | Sequence |
| SU | CTTAGTCTCCGCCTCCACTG |
| SD | CTCTCCACCCACCACCATAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 61 bp | + | + | - |
| TDF | TDR | 282 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
