| Gene | Lrrc8a |
| Chromosome | 2 |
| Deletion Start | 30,255,179 |
| Deletion End | 30,262,222 |
| Deletion Size | 7,044 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,255,505 | 30,255,523 | TGCGGTGTGCTACGAGAAC |
| TUP | 30,255,524 | 30,255,547 | CGCCTGCATTGGTTTGCCAAGTAC |
| TUR | 30,255,575 | 30,255,595 | GCTACAGGCCAGGAAGATGAG |
| TDF | 30,261,595 | 30,261,615 | GCTGCCAGGTTGATATGTCTC |
| TDP | 30,261,622 | 30,261,646 | CAGGTACTGGGTAGAGCCCACTGCC |
| TDR | 30,261,680 | 30,261,700 | CCTCCAGGATGGGTCTCACAG |
PCR Primers
| Primer | Sequence |
| SU | TTCTTGGTCTTCCTCACTGC |
| SD | ACACTGCCAGGGTGCTAAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12378A-A1 | VGB6 | C57BL/6NTac | |
| 12378A-B9 | VGB6 | C57BL/6NTac | |
| 12378A-G4 | VGB6 | C57BL/6NTac | |
| 12378A-H4 | VGB6 | C57BL/6NTac | |
| 12378A-F11 | VGB6 | C57BL/6NTac | |
| 12378A-F3 | VGB6 | C57BL/6NTac | |
