12383 Tbx5 Status: ES cell colonies screened / QC one positive
| Gene | Tbx5 |
| Chromosome | 5 |
| Deletion Start | 119,836,917 |
| Deletion End | 119,845,112 |
| Deletion Size | 8,196 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,837,541 | 119,837,559 | GGAACCAAGGCACCCAGAG |
| TUP | 119,837,560 | 119,837,582 | CCCGGCTGACTGGAAGTTGGTCC |
| TUR | 119,837,600 | 119,837,623 | GCCACTCAAATAACAGACCACAAG |
| TDF | 119,844,448 | 119,844,466 | GCTCAGTTGGCAGGGTCAG |
| TDP | 119,844,471 | 119,844,496 | TGGCCTTCCTCAGAAGGTGTTTGAGG |
| TDR | 119,844,535 | 119,844,555 | GCAGTGAGGGACAGGAAGAAC |
PCR Primers
| Primer | Sequence |
| SU | GGCATGGTCTCTTGGATTTG |
| SD | ACCCATAGCAGCACAATGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
