12385 Il23r Status: ES cell colonies screened / QC one positive
| Gene | Il23r |
| Chromosome | 6 |
| Deletion Start | 67,490,733 |
| Deletion End | 67,466,283 |
| Deletion Size | 24,451 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 67,490,172 | 67,490,193 | AAGCAAGGGTATGGTTGACATC |
| TUP | 67,490,142 | 67,490,167 | AGTTTCTGTTGGGAGCAGCTGTCTCC |
| TUR | 67,490,113 | 67,490,134 | CTGCCTCCTGTTATCTGTGTTC |
| TDF | 67,466,743 | 67,466,767 | TCATCTTAATGTTCCCACCATCTTC |
| TDP | 67,466,717 | 67,466,741 | TGTGGTGCCAAGCTGGATACTAAGC |
| TDR | 67,466,695 | 67,466,716 | CCCAATGGCCCATGTGTTAAAG |
PCR Primers
| Primer | Sequence |
| SU | AGAGTAGCTGCCTGATGAAGG |
| SD | TTAGCAATTTCCAGAAGGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
