12390 Atxn7 Status: Cancelled
| Gene | Atxn7 |
| Chromosome | 14 |
| Deletion Start | 14,013,087 |
| Deletion End | 14,013,650 |
| Deletion Size | 564 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 14,013,299 | 14,013,317 | CAGTCCCGAAGCGATGCTG |
| TUP | 14,013,321 | 14,013,348 | CAGTCGTGGAATCTGTGGGTGGAAGCTT |
| TUR | 14,013,370 | 14,013,388 | AGGAGGCGTGGACACTCAC |
| TDF | 14,013,473 | 14,013,494 | GAGATGCTATCTTCTGCTGGGT |
| TDP | 14,013,497 | 14,013,517 | CCACACCTACCCGGAGCGTGC |
| TDR | 14,013,531 | 14,013,551 | CAGGTCAGTGGCTAGGAGTGT |
PCR Primers
| Primer | Sequence |
| SU | GAGCAGAGAGGGAGGGACC |
| SD | TCCCTGCCTGCTTCCTATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
