12396 Hoxd4 Status: ES cell colonies screened / QC positives
| Gene | Hoxd4 |
| Chromosome | 2 |
| Deletion Start | 74,727,282 |
| Deletion End | 74,728,663 |
| Deletion Size | 1,382 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 74,727,297 | 74,727,320 | ATGGTGAACTCTAAGTACGTGGAC |
| TUP | 74,727,321 | 74,727,343 | CCCAAGTTTCCTCCGTGCGAGGA |
| TUR | 74,727,381 | 74,727,399 | CACCGCTGCCGTAGTAGTC |
| TDF | 74,728,484 | 74,728,505 | AGGGCAGGTCTTCTTCCTCATC |
| TDP | 74,728,507 | 74,728,528 | TCCTGCTCCTCTTCAGCTGCCC |
| TDR | 74,728,554 | 74,728,573 | GGTCCGTATGGTGGTCCTTG |
PCR Primers
| Primer | Sequence |
| SU | CACATTGCCCACACTCATTC |
| SD | TTGCACTGAAAGCACCTAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
