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12397 Bai3 Status: ES cell clone microinjected

Gene	Bai3
Chromosome	1
Deletion Start
Deletion End
Deletion Size	751
Genome Build	37

MGI ID	MGI:2441837
NCBI Gene ID	210933
BAC ID	378D16
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	No

Sequence Downloads and Strategy Information

2,000 Bases Upstream (not yet available)
2,000 Bases Downstream (not yet available)
Primer Sequences
Cassette
Design Diagram
PCR Conditions
Genotyping Strategy

Primer Genomic Coordinates on Chromosome 1

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	25,826,262	25,826,279	TGAGCCCGAGCCAGTTTG
TUP	25,826,234	25,826,259	TGCCATGTCTTATGCACTTGGTTGGA
TUR	25,826,191	25,826,210	TCCACAAGACTCGGTTCTCC
TDF	25,826,075	25,826,095	GAATGAGCAGACGGAGGGTTG
TDP	25,826,047	25,826,074	CCTGACTCAGGAGCTGCAAACTACTCAA
TDR	25,826,023	25,826,041	GGCCTCCCTGGTCAGATTG

PCR Primers

Primer	Sequence
SU	GAATGATAATGGATGTGCAGG
SD	CTGAGGTCTCATTTCTATCTC
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	89 bp	+	+	-
TDF	TDR	73 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+