| Gene | Eltd1 |
| Chromosome | 3 |
| Deletion Start | 151,439,288 |
| Deletion End | 151,439,385 |
| Deletion Size | 98 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 151,439,294 | 151,439,313 | TGTCTCCCCAATGCCAAGTG |
| TUP | 151,439,314 | 151,439,338 | CGAAGTGCACAATGGTGTGGAAGCC |
| TUR | 151,439,364 | 151,439,384 | CACAAATCGTGACGCCATTCC |
| TDF | 151,439,300 | 151,439,320 | CCCAATGCCAAGTGCGAAGTG |
| TDP | 151,439,321 | 151,439,347 | CACAATGGTGTGGAAGCCTGCTTCTGC |
| TDR | 151,439,359 | 151,439,377 | CGTGACGCCATTCCCAGAG |
PCR Primers
| Primer | Sequence |
| SU | GCTAGGAGAGTCATTTGAGG |
| SD | AGAAGTCAATAGAGGCTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12398A-A2 | VGB6 | C57BL/6NTac | |
