12403 Adamts5 Status: ES cell colonies screened / QC one positive
| Gene | Adamts5 |
| Chromosome | 16 |
| Deletion Start | 85,900,570 |
| Deletion End | 85,862,858 |
| Deletion Size | 37,713 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | TGGGCAGAGTATGAACGAATTTATG |
| TUP | | | ATGGATCTTCCCGCATCCTGCATGTC |
| TUR | | | TGCAGGAGTCTCGCAACTG |
| TDF | | | GCAAGGGTTATACTGACGTTGTG |
| TDP | | | AGGATCCCTGAAGGAGCAACCCACA |
| TDR | | | AGGGCTAAGTAGGCAGTGAATC |
PCR Primers
| Primer | Sequence |
| SU | CGTCTCTTGGACCTCCATTC |
| SD | GCTGTGTGTCATGGCTGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12403A-D8 | VGB6 | C57BL/6NTac | |
