| Gene | Adamts5 |
| Chromosome | 16 |
| Deletion Start | 85,900,264 |
| Deletion End | 85,862,552 |
| Deletion Size | 37,713 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 85,899,721 | 85,899,745 | TGGGCAGAGTATGAACGAATTTATG |
| TUP | 85,899,692 | 85,899,717 | ATGGATCTTCCCGCATCCTGCATGTC |
| TUR | 85,899,629 | 85,899,647 | TGCAGGAGTCTCGCAACTG |
| TDF | 85,863,156 | 85,863,178 | GCAAGGGTTATACTGACGTTGTG |
| TDP | 85,863,131 | 85,863,155 | AGGATCCCTGAAGGAGCAACCCACA |
| TDR | 85,863,073 | 85,863,094 | AGGGCTAAGTAGGCAGTGAATC |
PCR Primers
| Primer | Sequence |
| SU | CGTCTCTTGGACCTCCATTC |
| SD | GCTGTGTGTCATGGCTGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 117 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12403A-D8 | VGB6 | C57BL/6NTac | |
