12407   Bmp6     Status: Germline transmission achieved

GeneBmp6
Chromosome13
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:88182
NCBI Gene ID12161
BAC ID221P5
BAC LibrarybMQ
CassetteTM-ZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 13

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFCACATCAACGACACCACAAAGAG
TUPCAACCTATCCCAGATTCCTGAGGGTGA
TURGTCCTTGTAGACGCGGAACTC
TDFTCCCAGATTCCTGAGGGTGAG
TDPTGACGGCTGCTGAGTTCCGC
TDRCCACCACACAGTCCTTGTAGAC

PCR Primers

PrimerSequence
SUACTGCAGAAACTACAGTATCC
SDGTCAGAGTTGACTGGTCATC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTCATTCTCAGTATTGTTTTGCC
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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