| Gene | Gm889 |
| Chromosome | 12 |
| Deletion Start | 40,185,412 |
| Deletion End | 40,177,059 |
| Deletion Size | 8,354 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 40,184,497 | 40,184,520 | ACTATCCTAACACAGGCGTGAATG |
| TUP | 40,184,470 | 40,184,495 | CCCATTTGCCATTTGCCTCATGCTCA |
| TUR | 40,184,449 | 40,184,469 | AACTGGGCAGGAATTGTGGAG |
| TDF | 40,177,342 | 40,177,363 | GCACCGTTTCCGTTTCTTTCTG |
| TDP | 40,177,315 | 40,177,338 | TTCTCGGATGCATGGGATTTGCCA |
| TDR | 40,177,275 | 40,177,296 | GCCCTGATAGGCTACTCAACTG |
PCR Primers
| Primer | Sequence |
| SU | TTGCACTGAGGACTTGAAGC |
| SD | GATTCCTTAAGCTTGTGCCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12414A-D3 | VGB6 | C57BL/6NTac | |
| 12414A-E8 | VGB6 | C57BL/6NTac | |
