| Gene | Tmem179 |
| Chromosome | 12 |
| Deletion Start | 112,511,045 |
| Deletion End | 112,501,665 |
| Deletion Size | 9,381 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 112,510,943 | 112,510,961 | GAGAACGGCCACGACTTTC |
| TUP | 112,510,919 | 112,510,941 | TGGCCGCTGCCTACTCTTTACCG |
| TUR | 112,510,894 | 112,510,915 | AGGTTGGCACTCAGCCACATAC |
| TDF | 112,502,584 | 112,502,603 | CACTGCCTGTGGCTTTCATC |
| TDP | 112,502,561 | 112,502,582 | TCCGTGACACCCTCCCATGCTC |
| TDR | 112,502,509 | 112,502,531 | GGACCATCTCAGCCTAGTGTTTG |
PCR Primers
| Primer | Sequence |
| SU | GGCAAAGGAGTCTGAACTGC |
| SD | CCTTCTGAGAACCTTGGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12422A-A7 | VGB6 | C57BL/6NTac | |
| 12422A-B9 | VGB6 | C57BL/6NTac | |
| 12422A-C4 | VGB6 | C57BL/6NTac | |
| 12422A-E6 | VGB6 | C57BL/6NTac | |
