12424 4930578I06Rik Status: Cancelled
| Gene | 4930578I06Rik |
| Chromosome | 14 |
| Deletion Start | 63,987,744 |
| Deletion End | 63,971,136 |
| Deletion Size | 16,609 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 63,987,646 | 63,987,667 | CAGAGGATATGGTGGGAAGGTG |
| TUP | 63,987,619 | 63,987,643 | CCAACGAGGCTCGTGGATGCTGTCT |
| TUR | 63,987,583 | 63,987,606 | GATGATGTGCTGTGATGTTAGAGG |
| TDF | 63,971,282 | 63,971,301 | CTGGACCAGCAGCCTTAGAG |
| TDP | 63,971,249 | 63,971,276 | TCCCAAGGCTAGTAAGGCCAATAAAGGA |
| TDR | 63,971,195 | 63,971,215 | GCGTGTCCTTGAGTGGTAGTC |
PCR Primers
| Primer | Sequence |
| SU | ATTATGGAAGCCACTGGGTG |
| SD | ACTGAGCCATTGTCTCCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
