12434 4930420K17Rik Status: ES cell colonies screened / QC no positives
| Gene | 4930420K17Rik |
| Chromosome | 5 |
| Deletion Start | 9,101,321 |
| Deletion End | 9,118,577 |
| Deletion Size | 17,257 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 9,101,669 | 9,101,687 | GCACTTTCCAGCTCCACAC |
| TUP | 9,101,688 | 9,101,710 | TTCAGGTGTGCGTCCAGGGCTTT |
| TUR | 9,101,731 | 9,101,749 | GAAGCAACCCTGGGCTCTC |
| TDF | 9,117,843 | 9,117,861 | CTGCTGGGTCTGCTTTCAC |
| TDP | 9,117,865 | 9,117,891 | AGGAAACAGTCAAACCTTGGTGTGGCA |
| TDR | 9,117,910 | 9,117,930 | GCCCGAGCATATTAGATCACC |
PCR Primers
| Primer | Sequence |
| SU | CCGAGCTAGGGTGGTGTG |
| SD | AGATCTTCACAATCAAAGCTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
