12451 Cel Status: Germline transmission achieved
| Gene | Cel |
| Chromosome | 2 |
| Deletion Start | 28,419,107 |
| Deletion End | 28,411,581 |
| Deletion Size | 7,527 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,418,108 | 28,418,128 | AGTTCTCTGGCCCTTGTCTTG |
| TUP | 28,418,081 | 28,418,106 | TTTCTTGGACCCTGGAGACCATCCTG |
| TUR | 28,418,057 | 28,418,078 | AGGAGGGTGACACTGAGGATTC |
| TDF | 28,411,992 | 28,412,015 | CTCACCATGACTGAGCCTTGAAAG |
| TDP | 28,411,969 | 28,411,990 | TTGGCTCGGATGGCCAAGCTGT |
| TDR | 28,411,921 | 28,411,940 | GGCCTGCAAAGAGACTGGAG |
PCR Primers
| Primer | Sequence |
| SU | ACCTCAAGATGAAGCCCACC |
| SD | AGCTTTCTATAGCTGCTTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12451A-A2 | VGB6 | C57BL/6NTac | |
| 12451A-A7 | VGB6 | C57BL/6NTac | |
| 12451A-D2 | VGB6 | C57BL/6NTac | |
| 12451A-H7 | VGB6 | C57BL/6NTac | |
| 12451A-G4 | VGB6 | C57BL/6NTac | |
| 12451A-D3 | VGB6 | C57BL/6NTac | |
