12458 Chi3l3 Status: ES cell colonies screened / QC no positives
| Gene | Chi3l3 |
| Chromosome | 3 |
| Deletion Start | 106,167,518 |
| Deletion End | 106,147,790 |
| Deletion Size | 19,729 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 106,166,780 | 106,166,804 | CACTCTCCAATAGAAATCCAGCCAC |
| TUP | 106,166,752 | 106,166,773 | TGATAAGATGACCTGATTTACA |
| TUR | 106,166,654 | 106,166,678 | CAGTGCCACACAAATAGAATGTTTG |
| TDF | 106,148,565 | 106,148,589 | ACACAGTGCAAGTATGAGACTCTAC |
| TDP | 106,148,530 | 106,148,558 | ACTCATAGTGCATAATGCCCTGCATTCAC |
| TDR | 106,148,461 | 106,148,486 | GAAGAGCACATGAATGAATGGGAAAG |
PCR Primers
| Primer | Sequence |
| SU | TGCCAATATGGTCTTAGCTGG |
| SD | CCCATCCTCTTCATGTTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 151 bp | + | + | - |
| TDF | TDR | 129 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
