12459 Chi3l4 Status: Cancelled
| Gene | Chi3l4 |
| Chromosome | 3 |
| Deletion Start | 106,219,461 |
| Deletion End | 106,201,718 |
| Deletion Size | 17,744 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 106,219,416 | 106,219,440 | GGTGAGTTGATAATCTGATGCAGAC |
| TUP | 106,219,385 | 106,219,414 | TTCAGTCTTGCATGGTTTTTCCCTTACAGA |
| TUR | 106,219,351 | 106,219,372 | GAGCTGCAGTATACAAATTGCC |
| TDF | 106,202,471 | 106,202,495 | CACAGTGTATAATGCCCTTCATTCC |
| TDP | 106,202,452 | 106,202,469 | TCAAAGCCTGTCTGAATA |
| TDR | 106,202,330 | 106,202,352 | TCCCTGGTTCCTAATAAGTGTGC |
PCR Primers
| Primer | Sequence |
| SU | CCCTGGGAACTGCTAATATGG |
| SD | CCATCTCAAAGCCTGGAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 166 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
