12464 Ermap Status: ES cell colonies screened / QC positives
| Gene | Ermap |
| Chromosome | 4 |
| Deletion Start | 119,188,928 |
| Deletion End | 119,177,914 |
| Deletion Size | 11,015 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,188,306 | 119,188,325 | CCAGGCATCTACAGCCAGTG |
| TUP | 119,188,275 | 119,188,304 | TCGATGCATACTCTCCTCCTGAATCTGTCC |
| TUR | 119,188,250 | 119,188,268 | GGGCAGGGCTTCTTCACAG |
| TDF | 119,178,487 | 119,178,508 | AGTGAGTCTGTGAGCAGGAAAG |
| TDP | 119,178,465 | 119,178,484 | AAGGTCACGGCCTCGCCTGC |
| TDR | 119,178,406 | 119,178,425 | GGGACGTGAGAGCTTCATAC |
PCR Primers
| Primer | Sequence |
| SU | CACTTAAGGCAAGGCTGTGG |
| SD | GCGAGTTAGAGGCCTGACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
