| Gene | Foxo6 |
| Chromosome | 4 |
| Deletion Start | 120,287,249 |
| Deletion End | 120,267,814 |
| Deletion Size | 19,436 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,286,452 | 120,286,475 | GTCTGCTTTCCCTCTCAAATCTTC |
| TUP | 120,286,430 | 120,286,451 | CCTGGCCTTCCTGACACTGGCC |
| TUR | 120,286,400 | 120,286,422 | GGCTTGATCTGTTAGCACTCTTC |
| TDF | 120,268,007 | 120,268,030 | AACGACTTCATGGACAGTGACGAA |
| TDP | 120,267,978 | 120,268,002 | ACTTCAACTTCGACTCAGCCCTGCC |
| TDR | 120,267,924 | 120,267,942 | GGCACCCAGCTCTGGTTAG |
PCR Primers
| Primer | Sequence |
| SU | GGCAGGTGCTAGAAGCTGG |
| SD | GAGGACTGAGCAGAATGGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12465B-F6 | VGB6 | C57BL/6NTac | |
| 12465B-E5 | VGB6 | C57BL/6NTac | |
| 12465B-F9 | VGB6 | C57BL/6NTac | |
