12467 Gm13154 Status: ES cell colonies screened / QC no positives
| Gene | Gm13154 |
| Chromosome | 4 |
| Deletion Start | 147,577,103 |
| Deletion End | 147,584,437 |
| Deletion Size | 7,335 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | ACAGTGATGCTCAAAGAACAGAC |
| TUP | | | TGATGGGTATTTTCTATGTTG |
| TUR | | | ATGTGGCACAAGAGACCTTAC |
| TDF | | | TGCTTTACTGGAAAAGGCAGTCT |
| TDP | | | TCATCAGAGAATTCATACAGG |
| TDR | | | GCATTTGTCACATTCACTACATTTGTG |
PCR Primers
| Primer | Sequence |
| SU | TGTCATGAGAATTGGTGTTGC |
| SD | AGTGTTGCCAGGACACCATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
