12469 Phox2b Status: Cancelled
| Gene | Phox2b |
| Chromosome | 5 |
| Deletion Start | 67,098,938 |
| Deletion End | 67,096,038 |
| Deletion Size | 2,901 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 67,098,892 | 67,098,912 | TTCCTCTGCCTACGAGTCCTG |
| TUP | 67,098,869 | 67,098,890 | ATGGCCGGGATGGATACCTCCA |
| TUR | 67,098,804 | 67,098,825 | CGGGTTATACTGGAAGCCACTG |
| TDF | 67,096,894 | 67,096,914 | AGGGCCTTCCTGCTATCTTTG |
| TDP | 67,096,863 | 67,096,892 | ACTTGTCCCTTACGGTGTCATTTCTTTGGC |
| TDR | 67,096,811 | 67,096,830 | TCTCGCTGCTACTGCCTTTG |
PCR Primers
| Primer | Sequence |
| SU | TGATAGGGAGGTTGGACAGC |
| SD | GGGCCACAGACACACATTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
