| Gene | Zfp354c |
| Chromosome | 11 |
| Deletion Start | 50,826,455 |
| Deletion End | 50,814,472 |
| Deletion Size | 11,984 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 50,826,244 | 50,826,267 | CCTCCCTCCTCACACTTATCAAAG |
| TUP | 50,826,221 | 50,826,241 | TTCTGCCAGGCCCAGCACAGA |
| TUR | 50,826,197 | 50,826,219 | GGTCTTATGAGGTGGTGGCTATC |
| TDF | 50,815,140 | 50,815,159 | GTGGGAAGGGCTACAGTCAG |
| TDP | 50,815,115 | 50,815,137 | TCCATCCCTAGCCGAACACCAGA |
| TDR | 50,815,092 | 50,815,112 | AAGCTGCTCGCCAGTATGGAG |
PCR Primers
| Primer | Sequence |
| SU | TTGGTACCATTATGGGTGGG |
| SD | TTCCTCTGTAGTGGCTCCTTATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12482A-A7 | VGB6 | C57BL/6NTac | |
| 12482A-G3 | VGB6 | C57BL/6NTac | |
| 12482A-E7 | VGB6 | C57BL/6NTac | |
