| Gene | Nlrp3 |
| Chromosome | 11 |
| Deletion Start | 59,543,116 |
| Deletion End | 59,566,336 |
| Deletion Size | 23,221 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,543,128 | 59,543,146 | TGCAAGCTGGCTCAGTATC |
| TUP | 59,543,148 | 59,543,174 | AGAGGACCTTGAAGATGTGGACCTCAA |
| TUR | 59,543,194 | 59,543,213 | TTCTCGGGCGGGTAATCTTC |
| TDF | 59,565,890 | 59,565,911 | CACAATTCTGACCCACAACCAC |
| TDP | 59,565,916 | 59,565,936 | TTCGGAAGCTGAACCTGGGCA |
| TDR | 59,565,945 | 59,565,962 | CACGCACAGATCGCCAAG |
PCR Primers
| Primer | Sequence |
| SU | TTTCTTCCATGGCTCAGGAC |
| SD | TGAAGGTATGGGCAAAGGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12484A-F2 | VGB6 | C57BL/6NTac | |
| 12484B-H10 | VGB6 | C57BL/6NTac | |
| 12484B-C2 | VGB6 | C57BL/6NTac | |
| 12484C-D1 | VGB6 | C57BL/6NTac | |
