12486 Pafah1b1 Status: Cancelled
| Gene | Pafah1b1 |
| Chromosome | 11 |
| Deletion Start | 74,699,339 |
| Deletion End | 74,677,640 |
| Deletion Size | 21,700 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 74,699,226 | 74,699,247 | GGGTGGTAAAGACAAGCCCATC |
| TUP | 74,699,195 | 74,699,223 | TCATCTTCAGCCTTGGAAGTTCATGGCTA |
| TUR | 74,699,175 | 74,699,194 | GATTTCAGGTAGCCCAGGAC |
| TDF | 74,678,161 | 74,678,184 | ATGATGGATGGACCTTATGGTGAC |
| TDP | 74,678,131 | 74,678,157 | AAAGCCCTTGGTAAATTGGGCAGGAGT |
| TDR | 74,678,096 | 74,678,118 | TGAATGTTGTGCTTCAGGCTTAG |
PCR Primers
| Primer | Sequence |
| SU | CCAGGAGGATGCCTGTTCTAC |
| SD | AATGAGGCCATCAAGTCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
