| Gene | Fzd2 |
| Chromosome | 11 |
| Deletion Start | 102,604,735 |
| Deletion End | 102,606,497 |
| Deletion Size | 1,763 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,605,528 | 102,605,550 | TCACGGTCACCACCTATTTAGTG |
| TUP | 102,605,555 | 102,605,578 | TGCAGCGATTTCGCTACCCAGAGC |
| TUR | 102,605,586 | 102,605,605 | AGCAGCCGGACAGAAAGATG |
| TDF | 102,606,370 | 102,606,387 | GGCAAGACACTGCACTCG |
| TDP | 102,606,388 | 102,606,415 | TGGAGGAAGTTCTACACTCGTCTCACCA |
| TDR | 102,606,432 | 102,606,450 | ACCGCTTCACACAGTGGTC |
PCR Primers
| Primer | Sequence |
| SU | AGAGGAGGAAGAGGAGCTAG |
| SD | AATAGCGAGCAGGCTAGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 12490A-B2 | VGB6 | C57BL/6NTac | |
| 12490A-F1 | VGB6 | C57BL/6NTac | |
